Abstract
This study has been conducted in plant tissue culture laboratory in Aleppo Scientific Agriculture Research Center. Syria. the purpose is to find the best combinations to produce large number of shoot multiplication and develop suitable propagation method for three walnut cultivars ( Hartley. Serr. Blhsen2) cultivated in genetic centre of walnut at Gosea AL-Kharab agricultural research center in Homs to obtain the same genotype of the seedlings free from diseases. Axillary bud are used as initial explants in establishment culture. this explants were sterilized in weaponless chamber by several disinfection substances The results show that the best sterilization treatment with Hgcl2 0.1% at for 10 minutes. there was much variations in the influence of used growth regulators on number new formed axillary microshoots. Statistical analysis shows a great influence in interactions between cultivars and treatments. generally medium MS7 which was supplemented with 2 mg / l and 0.1 IBA. promoted the number of new formation microshoots and increased to report 5.6. 4.6. 5.2 with cultivars Serr. Hartley and Blhsen2 respectively. The medium MS3 supplemented only with 2 mg / l . as number of new formation microshoots was 4.2. 4.2. 4.4 to study cultivars respectively.
