Abstract
This investigation was carried out in the laboratory of cells and plant tissue culture at department of Horticulture and Landscape Design,College of Agriculture and Forestry,Mosul university,to propagate Calendula officinalis L. cv. Lemon Queen in vitro by multiplication shoot tips and nodes taken from field culture on MS medium supplemented with (0.0,0.5, 1.0, 1.5, 2.0) mg /l BA,shoot produced in vitro cultured on MS medium supplemented with (0.0,0.5, 1.0, 1.5, 2.0) mg/l IBA,moreover parts of leaves had been cultured on MS medium supplemented with 0.1 mg /l 2,4-D for callus initiation, callus cultured on MS medium supplemented with (0.0, 0.1, 0.5, 1.0) mg/l BA interacted with(0.0, 0.1, 0.5, 1.0) mg/l IBA for differentiation. Data refer that best multiplication 7.8 shoots/explants with 2.7 cm length were obtained from cultured shoot tip drived from field cultured on MS medium supplemented with 1.5 mg/l BA after eight weeks compared with nodes which gave 3.6 shoots / explants with 2.8 cm length from cultured on MS medium supplemented with 1.0 mg/l BA after eight weeks, highest rooting 70% with highest roots number 8.8 obtained from shoot tips produced in vitro cultured on MS medium supplemented with 0.5 mg /l IBA, highest fresh weight of callus 15.9 gm was initiated from callus cultured on MS medium supplemented with 1.0 mg /l IBA or from callus cultured on medium supplemented with 0.5 mg/l BA and 1.0 mg /l IBA, 4.0 shoot /explant regenerated from callus cultured on MS medium supplemented with 0.5 mg/l BA and 0.5 mg/l IBA, 10.2 root /explant obtained from callus cultured on MS medium supplemented with 0.1 mg /l BA after eight weeks.
