Abstract
This study showed the differentiation capability of dwarf Tagetes patula cv. nana callus to regenerated of plants directly and indirectly on in vitro aseptic medium, whereas was obtained of shoot organogenesis by two methods. The first by direct differentiation of callus which initiated on solidified MS medium containing 5mgL-1 of each of BA and IAA after 23 days of culture ,the second method was from indirect differentiation of callus on MS medium supplemented with 3mgL-1 BA with 0.5 mgL-1 NAA .The shoot arise after four days of transplanting of callus (21 days age) to same medium or to MS medium containing 5mgL-1 of BA with 0.1mgL-1 2,4-D. The producing shoots were readily rooted on MS medium containing 0.5mgL-1 of NAA. , and subsequently the plantlet producing from leaves callus were adapted(77.8%) to the environmental conditions through transplanting them in soil. they were showed faster growth in the first vegetative growth stage compared with seed plants.
