Abstract
In this study , thiophene which was a secondary metabolites product compound was isolated and detected from Tagetes patula plants, by using high performance liquid chromatography (HPLC). The results indicated the presence of high levels of thiophene in leaves, and roots of seedlings and callus of marigold plants, moreover, in callus culture and transformed hairy roots. High level of thiophene 40.01 and 44.54 mg/gm dry weight were recorded in the roots of plants produced from callus and the roots of seedling plants.
Interestingly, the results showed that transformed hairy roots by Agrobacterium rhizogenes R1601 bacteria showed high content of thiophene reached up to 34.14 mg/gm D.W as compared to 5.64 mg/gm D.W in leaves callus cultures. In other words, hairy roots showed an increased amount of thiophene equal 6 folds the amount of thiophene in callus tissue. Therefore transformed hairy roots can be good source to obtain the secondary metabolite thiophene and may be other products.
