Abstract
Biological activities of Citrus aurantifolial and Achillea Fragrantissmal extracts were tested against two species of Gram positive bacteria , Staph. lococcus aureus , Bacillus subtilis and four species of Gram negative bacteria , Pseudomonas aeruginosa , Proteus vulgaris , Kiebsilla pneumonia , Escherichia coli . Fruit of Citrus aurantifoliaL. and flowers of Achillea FragrantissmaL. were extracted using Dist water and Soxhlet apparatus in presence of Ethanol , Chloroform , and Aceton . Water extract of Achillea FragrantissmaL. showed no activity against all bacterial species tested , but the water extract of Citrus aurantifoliaL. showed a good antibacterial activities using disc diffusion method and comparing with the standard antibiotic (Gentamicin and Tetracycline ) . Result also indicated that ethanol extract showed high antibacterial activities in all types species tested . Chloroform extract of Citrus aurantifoliaL. and Acetone extract of Achillea FragrantissmaL.on the other hand, showed no activity on all species of bacteria tested , The study also detected the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of the two plant extracts , tested bacterial showed high sensitivity against acetone extract of Citrus aurantifoliaL. , Staph. aureus , B.subtilis , Pr.vulgris and E.coli was more sensitive than other tested bacteria , the calculated (MIC) reached 0.125 mg/ml while the ( MBC) reached 0.5 mg/ml .
