Abstract
This study was conducted to investigate the effect of some antioxidants ( Methionine , Vit.C and Vit.E ) in lipid peroxidation Malondialdehyde ( MDA ) and Glutathione ( GSH ) level in heart , kidney and pancreas tissues in laying hens . Hens ( 8 weeks old ) were divided into four groups ( 10 hens each – 2 replicates ) randomly , the 1 st group was given the standard ration , the 2 nd group was treated with methionine ( 450 mg/ kg. ration ) , the 3 rd group was treated with Vit.C ( 300 mg/ kg. ration ) and the 4 th group was treated with Vit.E ( 600 mg/ kg. ration ) daily for 12 weeks . At the end of the experiment , tissue samples were collected from heart , kidney and pancreas .Results showed that the treatment with antioxidants causes a significant decrease in lipid peroxidation MDA , and a significant increase in GSH levels in heart , kidney and pancreas . In conclusion , the study revealed that the antioxidants used in the study enhance the antioxidant status of the laying hens tissues .
