Abstract
This study was designed to determine the ability of hydrogen peroxide (0.5 %) consumed in drinking water to induce oxidative stress in lying hens and to make sure of oxidative stress interference occurred in creating fatty liver hemorrhagic syndrome (FLHS) in this pattern. Oxidative stress was determined through measuring the levels of glutathione (GSH) and malondialdehyde (MDA) in liver tissue and of Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) aspartate amino enzymes levels . H2O2 treatment showed significant increase in glucose , cholesterol and triglycerides levels and significant decrease in liver tissue glutathione GSH and asignificant increase in liver tissue malondialdehyde MDA . The levels serum AST and ALT were significantly increase also , the above changes indicate the ability of oxidative stress in laying hens . Methionine was used to determine its ability in preventing oxidative stress or to remove the effects of oxidative stress . The methionine was used as capsules given orally . Laying hens were divided into (5) groups . Each group included (7) birds were treated daily for (28) days . Methionine was chosen in order to test their ability to protect birds against oxidative stress or To remove the effect of oxidative stress as follow : 1- Treating the laying hens with methionine with out oxidative stress for 28 days . 2- Treating the laying hens with H2O2 together and methionine from beginning of treatment . 3- Stressing the laying hens from the beginning of treatment water with then treat it with methionine from the (15th) day of the beginning of H2O2 treatment . The treatment of methionine showed similar effects when it was used . It reduced the levels of glucose , cholesterol , triglycerides , ALT and AST in serum . Also the level of GSH increase whereas the level of MDA decreased , as well as decrease in the fat liver % . The results of this study were identical with the hypothesis that states : free radicals are considered strong causative factor in (FLHS) in laying hens so the H2O2 (0.5 %) induced oxidative stress in laying hens can be considered anew experimental pattern to be used in different scientific studies in order to create asimilar case of fatty liver hemorrhagic syndrome .
