Abstract
The effect of some growth regulators on the initiation , growth of (Gossypium hirsutun L.) callus and root / shoot formation was studied . The results showed that best medium callus for growth contain 0.5 mg/l BA with 1.0 mg/l NAA depending on callus fresh weight . The fresh weight reached 3.99g after 90 days of growth on their medium . Shoots formation occurred on medium containing 1.0 mg/l BA and 0.5 mg/l NAA after 80 days . However roots proliferation take place on medium containing 0.1 mg/l K and 1.0 mg/l IAA . The plants regenerated on particular medium was transferred to the soil after harding .