Abstract
Study were conducted to evaluate the biological effect of Beauveria bassiana with four concentrations (105, 106, 107 and 108) spores/ ml after two exposure times (one & two day) and two application methods as biocontrol agents against tomato leaf miner 1st instar larvae and its residual effect on other stages. Results indicated that B. bassiana was pathogenic to the first instar larvae of tomato leaf miner although. Its capability differed according to the application method, fungus concentration and exposure duration. The larval mortality percentages increased significantly with B. bassiana at 108 spores/ ml. to reach 58.146% and 40% for larvae directly sprayed and larvae feed on tomato leaves sprayed with B. bassiana conidial suspension respectively. The results also showed that B. bassiana had a residual effect on the survivous larvae after treatment, that the lowest adult emergency percentage was 0.00% from the first instar larvae treated directly with B. bassiana conidial suspension at both 107 and 108 spores/ml. compared with 96.66 % in control treatment.