Abstract
ELIZA test using antiserum to Watermelon mosaic virus (WMV) positive reaction with extract from squash plants showing mosaic, blistering and malformation leaves which indicate that the virus under study is WMV, the identification was confirmed by biological assay using quantitative indicator plant Chenopodium amaranticolor which respect chlorotic local lesions. The antibiotic Mitomycin, Para aminophenol, Chenopodium, alcoholic and aquatic extracts of thuja and chenopodium, and alcoholic extract of Eucalyptus at a high conc. showed a high inhibition on WMV in vitro when mixed with disease plant juice directly whereas the lowest inhibition effect resulted when used Bavastin at conc.0.5ml/L (3.8 spot / leaf). The alcoholic extract of Eucalyptus and Para aminophenol showed a high inhibitor effect on WMV in vivo, followed by alcoholic extract of Thuja and aquatic extract of Chenopodium, Bavastin and aquatic extract of Eucalyptus showed restricted effects on virus at 0.5ml /L and 12.5mg / ml respectively,. The higher inhibition of all treatments particularly at a high concentration were compatible with ELISA test results at 405nm.
