EFFECT OF DILUENT TYPE AND PRESERVATION PERIODS ON SOME SEMEN CHARACTERESTICS OF AWASSI RAMS.
Mesopotamia Journal of Agriculture,
2012, Volume 40, Issue 0, Pages 193-201
AbstractThe objective of this study was to evaluate some diluents and effects of type of diluents and preservation periods of Awassi ram semen. The diluents included egg yolk-sodium citrate, Tris fructose –egg yolk ,Skim milk-egg yolk and new modified diluents. Dilution rate was (1:10) and semen samples were collected with artificial vagina , one ejaculate per week for 3 months (September , October, and November) in the breeding season and preserved at 5oC for 4 days to evaluated the changes in daily spermatozoa, Individual motility , percentage of alive sperm, pH, and sperm abnormalities. Total ejaculates were collected from 6 Awassi Rams with weight mean 54 kg and aged 1.5- 2 years. Results of the present study showed:
1- Tris fructose – egg yolk diluent was preserved significantly (p ≤ 0,01) to the sperms in semen's diluent of Awassi Rams and was superior (p ≤ 0,01) than other diluents in spermatozoa individual motility, percentage of alive sperm than other dilutions.
2- Tris fructose – egg yolk and skim milk – egg yolk diluents were less significant (p ≤ 0,01) with degree of sperms abnormality in diluted semen than Sodium citrate (SC) and modified dilutions .
3- Decrease the efficiency of Modified dilution for preserving the sperms of diluted semen with keeping good level of acidity (pH) than other dilutions.
4- The preservation periods had a significant effect on diluted and cooling semen characteristics, however the Tris fructose – egg yolk was superior dilution for preserving Awassi semen at 5oC for 4 days.
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